Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry Scientific Notes of V.I. Vernadsky Crimean Federal University. Biology. Chemistry Ученые записки Крымского федерального университета имени В.И. Вернадского. Биология. Химия. 2413-1725 100800 10.29039/2413-1725-2025-11-1-273-282 ХИМИЧЕСКИЕ НАУКИ CHEMICAL SCIENCES ХИМИЧЕСКИЕ НАУКИ MONONUCLIAR COMPLEX OF ZINK WITH TETRADENTATE N2O2-DONOR LIGAND AS A PERSPECTIVE MATERIAL FOR BIOIMAGING МОНОЯДЕРНЫЙ КОМПЛЕКС ЦИНКА C ТЕТРАДЕНТАТНЫМ N2O2-ДОНОРНЫМ ЛИГАНДОМ КАК ПЕРСПЕКТИВНЫЙ МАТЕРИАЛ ДЛЯ БИОВИЗУАЛИЗАЦИИ Брага Е. В. Braga E. V. Мамонтов К. М. Mamontov K. M. Сидякин А. И. Sidyakin A. I. Гусев А. Н. Gusev A. N. Крымский федеральный университет имени В.И.Вернадского Crimean Federal University of a name of V.I.Vernadsky 01 07 2025 01 07 2025 11 1 273 282 01 07 2025 https://sn-biolchem.cfuv.ru/моноядерный-комплекс-цинка-c-тетраден/

Синтезирован и исследован люминесцирующий комплекс цинка на основе азометинового «salen-like» лиганда, который представляет собой две молекулы 3-метил-1-фенил-4-формилпиразол-5-она «сшитые» молекулой пропан-1,3-диамина. Состав и структура комплекса установлена по данным элементного, термогравиметрического анализа, ИК-спектроскопии. Молекулярная структура комплекса установлена на основе данных РСА. Комплекс проявляет интенсивную фотолюминесценцию в растворах и может использоваться для визуализации клеточных структур клеток эпидермиса Allium cepa.

Modern development of medicine, molecular biology and genetics is closely related to the improvement of methods for visualization of molecular processes occurring in the cells of living organisms. The fluorescence microscopy method, which provides high spatial resolution and good sensitivity, is particularly promising in this direction. Taking into account the instrumental capabilities and specificity of research objects, many commercial fluorophores based on organic dyes do not meet the high requirements of modern diagnostics, among which the most important are: ensuring specific binding to the target molecule, organelle or cell; high emission efficiency combined with photostability. In addition to the above-mentioned requirements, in the last decade the demand for phosphors sensitive to specific targets: biometals, pH of the medium, biomarkers has increased. The high luminescent activity of azomethine bases in the presence of zinc ions, combined with stability in solutions, sensitivity to the pH of the medium and observed two-photon luminescence, makes the proposed compounds ideal candidates for use in bioimaging. Zinc belongs to the group of trace elements, its uniqueness lies in the fact that it is a part of more than 300 enzymes and performs a variety of physiological functions, has antioxidant properties. Its toxicity for animals and humans is small — this is due to the fact that in excessive intake it is excreted by the body. Thus, zinc complexes luminescent in the visible region are promising dyes for biovisualization. A luminescent zinc complex based on azomethine “salen-like” ligand, which represents two molecules of 3-methyl-1-phenyl-4-formylpyrazol-5-one “cross-linked” by a molecule of propane-1,3-diamine, has been synthesized and studied. The composition and structure of the complex was established by elemental, thermogravitetric analysis and IR spectroscopy. The molecular structure of the complex was established on the basis of PCA data. The complex exhibits intense photoluminescence in solutions and can be used for visualization of cellular structures of A. cepa epidermis cells.

 люминесценция цинк биовизуализация luminescence zinc bioimaging.

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